Gradient Electrophoresis

Gradient Electrophoresis

The TV400-DGGE Denaturing Gradient Gel Electrophoresis System

The TV400-DGGE denaturing gradient gel electrophoresis system is a cost-effective solution for researchers studying mutations and DNA polymorphisms critical in disease aetiology and genetic diversity. Designed primarily for parallel denaturing gradient gel

electrophoresis (DGGE), where electrophoresis and the denaturing gradient run in the same direction, the TV400-DGGE has a maximum 96-sample throughput compatible with standard microplates and thermal cycler blocks. A 100ml gradient mixer is also included to pour gradient gels using our newly designed gel-running module and cam-caster, while a 400W heater, regulated by an external temperature control unit connected to a heat sensor within the gel tank, allows the gel temperature to be set to the predetermined melting temperature (Tm) of the PCR®- amplified DNA polymorphism or mutation of interest.

Benefits include:

• Maximum 96-sample throughput - using two 48-sample combs - allows samples to be transferred quickly and easily to the each gel from standard 96-well microplates or thermal cycler blocks
• Large format - 20.5 x 20cm glass plates allow gradient gels to be poured containing wider ranges of denaturant concentration, maximising sensitivity and resolution
• 100ml gradient mixer - used with the new style TV400-GRM and casting base to make two 1mm parallel denaturing gradient gels
• SERVA formamide, urea and acrylamide available - to pour denaturing gradient gels
• Can also be adapted for constant denaturing gel electrophoresis (CDGE) - if the denaturant concentration required for partial melting of the DNA target sequence is already known
• 400W heating element - manually controlled by a temperature control unit connected to a PT100 heat sensor - enables the gel and buffer temperature to be set in 1°C increments to a predetermined Tm, to a maximum 70°C

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Replacement Parts & Accessories

Gradient Mixers

Available in 15, 25, 50, 100 and 500ml volumes, Scie-Plas' gradient mixers are ideal for pouring polyacrylamide gradient gels and centrifugation gradients using sucrose or caesium chloride solutions. Each gradient mixer is precision engineered from high quality acrylic plastic and comprises leak-free valves that control mixing between the reservoir and mixing chambers and the resultant output flow.

Benefits include:

• Available in 5 different sizes - for different applications
• GM15 and GM25 - 15 and 25ml volumes for sucrose and caesium chloride gradients; the GM25 can also make two 1mm thick, 10 x 10cm gradient gels in the TV100-MC2
• 2-gel multicaster
• GM50 - 50ml volume sufficient to pour up to five 1mm thick, 10 x 10cm gradient gels or one standard 1mm thick, 20.5 x 20cm gel using the TV400 casting base
• GM100 - 100ml volume for pouring up to ten 1mm thick, 10 x 10cm gels in the TV100-MC10 10-gel multicaster or two 1mm thick, 20.5 x 20cm gels using the TV400-MC2 2-gel multicaster
• GM500 - 500ml volume suitable for up to ten 1mm thick, 20.5 x 20cm gels in the TV400-MC10 10-gel multicaster
• Reservoir chamber - designed to contain higher percentage acrylamide solution when making gradient gels with the TV100 and TV400 multicasters
• Mixing chamber - each is of sufficient diameter to accommodate a magnetic stirrer to mix lower percentage acrylamide with higher percentage acrylamide from the reservoir chamber
• Valves - control output flow from both the reservoir and mixing chambers
• Flat base - allows each gradient mixer to be placed onto a magnetic stirring plate
• Support rod - secures each gradient mixer to a retort stand
• Can be used in conjunction with the Buffer Recirculation Pump

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TV100, TV200 and TV400 Gel Multicasters

Scie-Plas now offers 2 and 10 gel multicasters for use with its 10 x 10cm (W x H) TV100 mini-gel, 20.5 x 10cm TV200 wide format mini-gel and 20.5 x 20cm TV400 maxi-gel units. Gels can be poured either as gradient gels with the hose connected to the

inlet port at the bottom of the front plate, or as standard polyacrylamide gels from the top of the casting chamber using a pipette

Benefits include:

• Available in 6 different formats: -
• TV100-MC2 - casts two 1mm thick, 10 x 10cm standard or gradient mini-gels
• TV100-MC10 - casts up to ten 1mm thick, 10 x 10cm standard or gradient mini-gels
• TV200-MC2 - casts two 1mm thick, 20.5 x 10cm standard or gradient wide format mini-gels
• TV200-MC10 - casts up to ten 1mm thick, 20.5 x 10cm standard or gradient wide format mini-gels
• TV400-MC2 - casts two 1mm thick, 20.5 x 20cm standard or gradient maxi-gels
• TV400-MC10 - casts up to ten 1mm thick, 20.5 x 20cm standard or gradient maxi-gels
• Dovetailed silicone gasket - acts in conjunction with 5 nylon screws to distribute pressure evenly across the glass plates for leak-free casting
• Separation sheets - 1mm thick - for easy separation of each gel cassette after casting and to maintain the correct pressure between the glass plates and the front plate of the casting chamber, ensuring that straight gels, of uniform thickness, are poured
• V-shaped cavity - stabilises the gradient in the bottom of casting chamber
• Inlet ports - maintain the steady flow of acrylamide into the casting unit before polymerization

Ordering information:

Replacement Parts & Accessories