Scie-Plas Blotting Units

Scie-Plas Blotting Units

TV100-EBK Mini and TV400-EBK Maxi Electroblotters

Recently redesigned to accommodate up to 4 electroblotting cassettes, the Scie-Plas TV100-EBK and TV400-EBK electroblotters provide a cost-effective and safe and easy to use system for the transfer of proteins and nucleic acids from 10 x 10cm mini and

20.5 x 20cm maxi gels. Both units are supplied with asymmetric colour-coded cassettes as a unique design feature to prevent them from being inserted into the tank in the wrong orientation, against the direction of transfer.

Benefits include:

• Available in 2 different formats: -
• TV100-EBK - accommodates a maximum of four 10 x 10cm electroblotting cassettes
• TV400-EBK - accommodates a maximum of four 20.5 x 20cm electroblotting cassettes
• Asymmetric, colour-coded cassettes - hinged, with red and black covers corresponding to the anode and the cathode, to ensure correct transfer
• Fibre pads - compress the gel against the nitrocellulose membrane for uniform transfer
• Plate-electrodes - 12.8cm apart - maximise the intensity of the electric field, allowing rapid transfer within 4 to 6 hours
• Enhanced cooling - included as standard - snap-lock connectors allow the cooling coil within each unit to be connected to the JULABO chiller units for faster, high intensity, transfers, or extended overnight runs without buffer depletion
• Flat bottom - allows each electroblotter to be placed directly onto a magnetic stirring plate
• Blotting paper, membranes and SERVA chemicals also available

Ordering information:

Replacement Parts & Accessories

TV Complete Modular Systems

The TV complete modular systems provide a fully integrated solution within one gel tank for both two dimensional (2-D) electrophoresis and electroblotting. These systems are available in existing TV100, TV200 and TV400 formats and comprise individual gel-running modules designed specifically for denaturing or native PAGE (GRM), capillary gel isoelectric focusing (CAPGRM) and electroblotting (EBGRM). Following 2-D

electrophoresis, the EBGRM can be used to transfer second dimension SDS-PAGE gels for further analysis by western blotting, providing enhanced sensitivity.

Benefits include:

• Comprises - one standard GRM, one CAPGRM and one EBGRM, which are interchangeable within the same gel tank, allowing the user to perform PAGE, IEF and electroblotting either independently or as part of a fully integrated system
• CAPGRM - for first dimension electrophoresis of up to 10 ampholyte capillary gels, a maximum 8 or 17cm in length and 1 or 4mm in internal diameter
• Twin-plate standard GRM - designed specifically for SDS-PAGE techniques requiring self-cast gels, although commercially available 10 x 10cm pre-cast gels may be used in the TV100-GRM
• Twin-cassette EBGRM - allows simultaneous transfer of 2 polyacrylamide gels in colour-code electroblotting cassettes, while plate electrode only 6cm apart maximise the intensity of the electric field
• Casting base and cooling - included as standard - act in tandem with glass plate stops within the GRM to lock the plates firmly in position on the casting base, whereas snap-lock connectors allow the cooling coil within the tank t be connected to the JULABO chiller unit for high voltage/current separations
• Colour-coded combs and spacers - available in 1 and 1.5mm thicknesses
• Nitrocellulose and PVDF membrane available plus SERVA 10 x 10cm precast gels, ampholytes, 2-D and PAGE chemicals

Ordering information:

Contact Progen Scientific for all accessories or copy of catalogue

TV Modular Electroblotting Systems

The TV modular electroblotting systems provide a complete solution within one gel tank for PAGE and electroblotting. These systems are available in existing TV100, TV200 and TV400 formats, and comprise individual gel-running modules designed specifically for PAGE (GRM) and electroblotting (EBGRM).

Benefits include:

• Plate-electrodes - only 6cm apart - maximise the intensity of the electri field, allowing rapid transfer in as little as 1 hour
• Colour-coded electroblotting cassettes - corresponding to the anode and cathode - ensure that transfer is performed in the correct orientation, while fibre pads compress the gel against the membrane for uniform transfer
• Twin-plate standard GRM - designed specifically for SDS-PAGE techniques requiring self-cast gels, although commercially available 10 x 10cm pre-cast gels may be used in the TV100-GRM
• Casting base and cooling - included as standard - act in tandem with glass plate stops within the GRM to lock the plates firmly in position on the casting base, whereas snap-lock connector allow the cooling coil within the tank to be connected to the JULABO chiller unit for high voltage/current separations
• Colour-coded combs and spacers - available in 1mm thickness
• Nitrocellulose and PVDF membranes available plus SERVA 10 x 10cm precast gels and blotting buffers

Ordering information:

TV Modular 2-D Systems

The TV modular 2-D systems provide a complete solution within one gel tank for two-dimensional (2-D electrophoresis. These systems are available in existing TV100, TV200 and TV400 formats and comprise individual gel-running modules designed specifically for denaturing or native PAGE (GRM) and isoelectric focusing (IEF) with capillary gels (CAPGRM).

Benefits include:

• Capillary gels - available in standard 1mm internal diameter for analytical IEF, or as optional 4mm capillaries for protein isolation
• Gel extraction platform (GEP) - for safe and easy collection and transfer of fragile, extruded capillary gels
• Twin-plate standard GRM - designed specifically for SDS-PAGE techniques requiring self-cast gels, although commercially available 10 x 10cm pre-cast gels may be used in the TV100-GRM
• Casting base and cooling - included as standard - act in tandem with glass plate stops within the GRM to lock the plates firmly in position on the casting base, whereas snap-lock connectors allow the cooling coil within the tank to be connected to the JULABO chiller unit for high voltage/current separations
• Colour-coded combs and spacers - available in 1 and 1.5mm thicknesses the latter suited to 1.5mm thick capillary gels for second dimension 2-D electrophoresis
• SERVA ampholytes, 2-D and PAGE chemicals available

Ordering information:

Contact Progen Scientific for all accessories or copy of catalogue

The TV100YK and TV400YK-2D-IEF-SYS Units: Dedicated 2-D Electrophoresis Systems

Scie-Plas now provide the first of a series of innovative solutions for scientists who prefer the latest advances in IPG (immobilized pH gradient) strip technology to more traditional capillary gels as the first step of 2-D electrophoresis. Their recently improved flatbed

isoelectric focusing system (IEF-SYS) allows 1st dimension 2-D electrophoresis to be performed with multiple IPG strips within the same tank, while 2nd dimension SDS-PAGE is carried out in their existing TV100 and 400 Mini- and Maxi-gel formats

(TV100YK or TV400YK). Scie-Plas offer the complete 2-D electrophoresis system with everything you need for running reproducible 2-D gels.

IEF-SYS for the 1^st dimension:

A recently redesigned IEFSYS unit where a maximum of 30 SERVA IPG strips, each 3mm wide and up to 24cm in length, may be run simultaneously on a 27 x 27cm ceramic cooling plate.

Benefits include:

• Glass electrode frame - sits flush with the ceramic cooling plate for optimal cooling efficiency
• Positive and negative electrodes - made from clear acrylic clip neatly within a glass electrode frame that allows the voltage gradient to be fine tuned along the entire length of each IPG strip
• Glass electrode support slab - ensures that the electrodes remain in complete contact with the positive and negative ends of each IPG strip during IEF

For the 2^nd dimension:

Either the TV100YK twin-plate 10 x 10cm (W x H) mini-gel unit or the TV400YK twin-plate 20.5 x 20cm (W x H) maxi-gel unit

Benefits include:

• Casting & cooling included as standard
• New-style GRM for leak-free casting

Adaptable either for 1mm or 1.5mm thick gels in the 2nd dimension

EV232 High-Voltage 3kV Power Supply

The Consort EV232 power supply - 3000V, 150mA, 150W

Benefits include:

• Nine different program settings - each with 9 different parameters - allow more complex, multi-step techniques to be programmed and stored, while voltage ramping, automatic crossover and recovery after power failure are included as standard
• High voltage 3kV output - allows desired Volt-hours to be attained faster at maximum voltage during IEF resulting in shorter overall run time
• 4/2MMA adaptors - compatible with the 2mm recessed outputs of the power supply - adapt the 4mm shrouded power connectors of the TV100YK and TV400YK units for lower voltage 2nd dimension SDS-PAGE

JULABO FL300 Chiller maintains cooling plate temperature during IEF

Benefits include:

• Temperature range: -20 to +40°C
• Can be easily adjusted to +20°C - the ideal temperature for running IPG strips
• Flow rate 15L/min / Maximum pressure 350mbar

Ordering information:

Contact Progen Scientific for all accessories or copy of catalogue