Labnet Enduro Modular Vertical PAGE Electrophoresis System

Labnet Enduro Modular Vertical PAGE Electrophoresis System

Labnet Enduro™ Modular Vertical PAGE System

Labnet's Enduro™ Vertical PAGE System is modular, allowing PAGE electroblotting and 2D gel electrophoresis to be carried out in the same tank, simply by changing the inserts. Users can purchase only the parts they need, or a complete combined system for a cost saving over buying all three systems separately.

Features

• Modular design - all three inserts, PAGE, electroblotting and 2D electrophoresis, have their own electrode assembly that connects into the lid of the buffer tank
• Most pre-cast gels, including NuSep™, Bio-Rad™, Invitrogen™ and Cambrex™, can be used with the Enduro™ PAGE system. The use of pre-cast gels saves time by eliminating the need to pour gels and wait while they polymerise
• Built for safety and endurance - the buffer tank is moulded from high quality acrylic for leakproof performance and long service life. Pins on the top of the inserts ensure that the lid is placed onto the insert in the proper orientation. These pins also aid in the removal of the lid. Removing the lid disconnects power to the electrodes
• Quick and easy set-up

PAGE Insert: A set of plates are placed on either side of the insert and held into place by the pressure bar. An additional gel may be run on each side by stacking the plates. The assembly is then placed into the casting stand for pouring the gel. After the gel is polymerised, the insert is removed from the casting stand and placed in the buffer tank

Electroblotting Insert: The electroblotting insert has a capacity for 3 gels. Setting up blots is easy - gels are sandwiched in the blotting cassettes with the transfer membranes. The cassettes are then placed in the insert, which is in turn placed in the buffer tank. Coiled electrodes in the insert generate a high intensity current, allowing transfer to take place in about an hour

2D Electrophoresis Insert: This insert is used for running the first dimension of IEF gels. Up to 10 x 8cm. gels are poured in disposable capillary tubes and placed in this insert. Graduated ports allow for easy insertion and removal of tubes. Blocking plugs are included for closing off ports that are not in use

• Gel Casting:

• 1. Put together bonded spacer plain glass plate with notched plate
• 2. Insert inside pressure bar with module on
• 3. Fully tighten screws ensuring not to wobble unit
• 4. Insert onto casting base and turn cams inwards
• 5. Pour resolving gel and allow to set, then stacking gel solution and insert comb
• 6. Once set, transfer to tank and fill inner and outer chambers

• Running a PAGE gel:

• 1. Insert PAGE module into tank
• 2. Cover with 250 ml. of Tris glycine buffer
• 3. Connect electrodes to power supply
• 4. 150 volts for 90 minutes

• Wide range of accessories - a variety of combs and spacers of varying widths are available to customise your Enduro™ system. All are colour coded and clearly marked for easy identification. Both notched and plain plates can be purchased with spacers permanently bound for easy set-up. Additional fibre pads and replacement cassettes are also available as well as capillary tubes, blanking ports and tube supports. Cooling packs are also available to help reduce buffer temperature during higher voltage or longer runs
• Enduro™ Packages - four Enduro™ packages are available.

1. The Enduro™ PAGE System includes the PAGE insert, casting stand, plates, combs and buffer tank.

2. The Enduro™ PAGE Electroblotting System includes all of the components of the PAGE System plus the electroblotting insert, three cassettes and six fibre pads.

3. The Enduro™ PAGE 2D Electrophoresis System includes all of the components of the PAGE System plus the tube gel insert.

4. An electroblotting package is also available that includes the buffer tank, electroblotting insert and accessories.

All components are also available separately

Ordering information

Additional spacers, combs and plates are available. Contact Progen Scientific.