Halo DB-20 UV / Visible Double Beam Spectrophotometer

The Halo DB-20 is a high performance double beam spectrophotometer suitable for many analytical applications where a higher level of accuracy is required.

 

True double beam optics ensure concurrent measurement of the sample and reference for improved stability, accuracy and reproducibility. The light beam is split in two using a half mirror so that one beam passes through the sample whilst the other passes through the reference side. Both beams are then measured on individual detectors. The reference side beam also acts to stabilise photometric values in a similar manner to the ratio beam principle.

 

Coma aberration elimination from the concave diffraction grating achieves a high resolution 1.5 nm. spectral bandpass and certifies compliance of the Halo DB-20 to the stringent European Pharmacopoeia standards.

Other specifications include an impressive wavelength accuracy of ± 0.3 nm., noise level 0.0003 Abs (500 nm.) and stray light ≤ 0.05% (220 nm. NaI, 340 nm. NaNO2)

 

Photometry Mode:Perform quantitative analyses in either absorbance or transmittance modes. Select from single wavelength, up to 6 individual wavelengths, nucleic acid / protein A260/A280 ratios and set up calibration curves with up to 20 standards for concentration measurements.

Time Scan:Perform kinetic measurements for time periods ranging from 1 minute to > 27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set.

Wavelength Scan:Perform a full spectral scan from 190 to 1100 nm. at any of 8 incremental and preset selectable selectable scan speeds starting from a high resolution 10 nm. / minute up to a swift 3600 nm. / minute. Data is displayed as either numerical values or a graphical spectrum. Furthermore, perform downstream processing of data, such as peak / valley search or smoothing, directly on board or with the optional Halo UV Detective software.

 

By virtue of the halogen tungsten and deuterium lamps typically found in higher end, analytical spectrophotometers, the Halo DB-20 wavelength range is an impressive 190 to 1100 nm. Furthermore, the dual lamp system results in higher accuracy then corresponding xenon lamps. Lamp switching is automatic and selectable from a wavelength range of 325 nm. to 370 nm. and both lamps are long life.

 

The extra large 190 mm. x 138 mm., backlit LCD screen with adjustable brightness control displays a large array of data also in graphical format. The seamless and chemical resistant keypad is designed for easy and quick selection of navigation and function features whilst protecting against any laboratory spills. Other unique features include the ‘GO TO WL’ short cut key to allow direct input of a new wavelength into an existing measurement.

 

To ensure optimum instrument performance, a self-diagnostic function incorporating a number of parameters and wavelength calibration are automatically initiated upon start-up. Furthermore, the Halo DB-20 is equipped with a GLP / GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can be all validated and the audit report printed.

 

The Halo DB-20 is fully equipped and capable of executing all functions in stand alone mode. Simply connect a standard laser printer for direct printouts of data and graphs. For more advanced analyses and reporting, the single slide of a switch places the Halo DB-20 under the direct control of the optional UV Detective™ software installed on a computer with Windows® XP Pro operating system (see separate specifications).

 

Up to 80 operating programmes and up to 10 sets of measurement data can be stored in the flash memory of the Halo DB-20. Programmes can easily be recalled, edited and deleted. Furthermore, when in stand alone mode, data (in text format) can be downloaded directly to an external memory stick via the USB port and transferred for further processing to any computer loaded with commercial spreadsheets (such as Microsoft® Eccel).

 

Halo UV Detective™ is powerful, user friendly software specifically designed for the control (and data processing) of selected Halo spectrophotometers from computers installed with the Windows® XP Pro operating system.

The versatile UV Detective™ can control all spectrophotometer operations such as photometry, wavelength scans, time scans and more. Further functions include storage of methods programme, saving of numerical and graphical data, downstream data processing, data transfer to commercial spreadsheets such as Microsoft™ Excel and report generation.

Rectangular long-path cuvette holder: Designed for low concentration or low absorbance samples

Cylindrical long-path cuvette holder: Designed for low concentration or low absorbance samples using a cylindrical cuvette.

Thermostatic cuvette holder: Designed for applications requiring incubation and / or maintenance of a sample at a constant temperature.

5-Cuvette holder / changer: Designed for mounting up to 5 standard 10 mm. cuvettes on the sample beam side.

6-Cuvette holder / changer with electronic temperature control: Designed for mounting up to 6 standard 10 mm. cuvettes on the sample beam side with temperature control and stirring.

Micro-cuvette holder: Designed for measuring micro-volumes with 50μl. micro-cuvette.

Micro-cuvettes: Suitable for use in the micro-cuvette holder.

Auto Sample sipper (without temperature control): Designed for the rapid measurement of multiple or large amounts of sample without the requirement for manual washing or changing of cuvettes. Sample is sipped from an external tube directly into the sipper’s integrated cuvette and automatically measured. The sample can also be recovered post-measurement.

Auto Sample sipper (with temperature control): The same features as the Auto sipper with the added convenience of electronic temperature control to maintain the flow cuvette at a constant temperature.

Designed for the continuous measurement of trace samples. The sample can be injected into the flow cuvette with a syringe or other injection device.

Designed for measuting the transmittance / absorbance of glass samples or filters.

Designed for measuting the transmittance / absorbance of thin film-like samples.