Halo RB-10 UV / Visible Ratio Beam Spectrophotometer

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Halo RB-10 UV / Visible Ratio Beam Spectrophotometer

The Halo RB-10 is a ratio beam spectrophotometer with an extensive array of built-in functions for versatility and suitability to many analytical and biological applications. The ratio beam optics impart greater accuracy and reproducibility than the conventional, single beam optics.

Ratio Beam Optics

The absorbance signal in conventional single beam instruments can destabilise even during simple applications thus resulting in data inaccuracy. By contrast, ratio beam optics compensate and stabilise any signal fluctuations to increase accuracy and reliability even over prolonged usage such as in time course and kinetic measurements. The ratio beam principle involves splitting the beam generated by the light source by a half mirror. One of the split beams passes through the sample and is quantified by the detector whereas the other split beam, which is representative of the absorbance signal, is measured by an independent detector to obtain a signal reference. The ratio of the values from both detectors is then calculated to detect and compensate for any aberration in the energy of the light source or a temporal change of the optical elements and produce the highly stable photometric values.

Spectral Features

Boasting a 4 nm. spectral bandpass the Halo RB-10 offers superior spectra and peak resolution. Other specifications include an impressive wavelength accuracy of ± 0.5 nm., noise level 0.0005Abs (500 nm.) and stray light ≤ 0.05% (220 nm. NaI, 340 nm. NaNO2).

Built-in and Diverse Range of Measurement Modes

Photometry Mode:Perform quantitative analyses in either absorbance or transmittance modes. Select from single wavelength, up to 6 individual wavelengths, nucleic acid / protein A260/A280 ratios and set up calibration curves with up to 20 standards for concentration measurements.
Time Scan:Perform kinetic measurements for time periods ranging from 1 minute to > 27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set.
Wavelength Scan:Perform a full spectral scan from 190 to 1100 nm. at any of 8 incremental and preset selectable selectable scan speeds starting from a high resolution 10 nm. / minute up to a swift 3600 nm. / minute. Data is displayed as either numerical values or a graphical spectrum. Furthermore, perform downstream processing of data, such as peak / valley search or smoothing, directly on board or with the optional Halo UV Detective software.

Dual Lamp Advantage

By virtue of the halogen tungsten and deuterium lamps typically found in higher end, analytical spectrophotometers, the Halo RB-10’s wavelength range is an impressive 190 to 1100 nm. Furthermore, a dual lamp system results in higher accuracy then corresponding xenon lamps. Lamp switching is automatic (by default at 340 nm.) and both lamps are long life.

User friendly operation and information rich LCD display

The 94 mm. x 70 mm., backlit LCD screen with adjustable brightness control is sufficiently large to display a large array of data even in a graphical format. Furthermore, single wavelength absorbance or transmittance data can be enhanced and enlarged using the unique zoom function. The seamless and chemical resistant keypad is designed for easy and quick selection of navigation and function features whilst protecting against any laboratory spills. Other unique features include the ‘GO TO WL’ short cut key to allow direct input of a new wavelength into an existing measurement.

Validation Functions

To ensure optimum instrument performance, a self-diagnostic function incorporating a number of parameters is executed each time the Halo RB-10 is switched on. Furthermore, the Halo RB-10 is equipped with a GLP / GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can be all validated and the audit report printed.

Stand alone or PC Operation

The Halo RB-10 is fully equipped and capable of executing all functions in stand alone mode. Simply connect a standard laser printer for direct printouts of data and graphs. For more advanced analyses and reporting, the single slide of a switch places the Halo RB-10 under the direct control of the optional UV Detective™ software installed on a computer with Windows® XP Pro operating system (see separate specifications).

4 Sample Cuvette Holder

A cuvette holder / changer with a 4 cuvette capacity is supplied as standard. Therefore, measurement can be expedited by inserting the 4 cuvettes in tandem and manually sliding the holder / changer forwards or backwards to select the appropriate cuvette for measurement. The cuvette holder / changer is easily removed for cleaning purposes.

Halo UV Detective™ Software

Halo UV Detective™ is powerful, user friendly software specifically designed for the control (and data processing) of selected Halo spectrophotometers from computers installed with the Windows® XP Pro operating system.
The versatile UV Detective™ can control all spectrophotometer operations such as photometry, wavelength scans, time scans and more. Further functions include storage of methods programme, saving of numerical and graphical data, downstream data processing, data transfer to commercial spreadsheets such as Microsoft™ Excel and report generation.
Compatible Spectrophotometers
Halo RB-10 (optional), Halo DB-20 (optional), Halo DB-30 (standard)
Control Functions
Wavelength setting, auto-zero, auto calibration, optical path calibration, accessories such as 6-cuvette positioner and sipper
Measurement Conditions
Start-up, setting, output and storage of measurement parameters
Measurement Function
Wavelength scan, time scan, quantitative analysis, multi-spectrum measurement, kinetic analysis, concentration measurement and nucleic acid / protein measurements
Data Output
Display of spectra, data and scans (time and spectrum)
Quantitative Methods
Multi-wavelength, input of constant, standard curve calibration (linear, quadratic, cubic and segment)
Data Processing
Integral, derivative, flatness, calculation (spectrum and constant) and kinetic
RB-10 Accessories
Rectangular long-path cuvette holder: Designed for low concentration or low absorbance samples
  • Accomodates 4 x long-path cuvettes
  • Accepts cuvettes with 6 optical path lengths of: 10, 20, 30, 40, 50 & 100 mm.
Thermostatic cuvette holder: Designed for applications requiring incubation and / or maintenance of a sample at a constant temperature.
  • External water circulation maintains temperature stability
  • Operating temperature range: ambient to +40°C
  • Temperature stability: ± 0.3°C
  • Complete with tubing for quick connection to water source (such as circulating water bath)
Test tube holder: Designed for the direct measurement of samples in a test tube with no need to transfer to a cuvette.
  • Spring mechanism automatically adjusts and accepts test tubes with diameters 12 – 18 mm.
  • High ceiling cover accommodates even the tallest test tubes
Micro-cuvette holder: Designed for measuring micro-volumes with 50μl. micro-cuvette.
  • Wavelength range: 220 to 950 nm.
  • Noise level: ± 0.004 Abs (with 50μl. volumes)
Micro-cuvettes: Suitable for use in the micro-cuvette holder.
  • Made from quartz
  • Available size: 50μl.
Sample sipper: Designed for the rapid measurement of multiple samples. Sample is sipped from an external tube directly into the sipper’s integrated cuvette and automatically measured. The sample can also be recovered post-measurement.
  • Minimum sample volume: 0.6 ml.
  • Carryover: ≤ 1%
  • Sipper cuvette capacity: approx. 50μl.
  • Optical path length: 10 mm.
Concave diffraction grating / Ratio beam principle
Wavelength range
190 nm. – 1100 nm.
Spectral bandwidth
4 nm.
Stray light
≤ 0.05% (220 nm. NaI, 340 nm. Na NO2)
Wavelength accuracy
± 0.5 nm.
Photometric range
Absorbance: -3 to +3
%T: 0% to 300%
Concentration: 0.000 to 9.999
Wavelength scan speed
10, 100, 200, 400, 800, 1200, 2400, 3600 nm. / minute
Baseline stability
0.001 Abs / hr. (500 nm. after 2 hours)
Noise level
0.0005 Abs (500 nm.)
Light source
Tungsten-Halogen and Deuterium lamps
Light source switching
Automatic switching at 340 nm.
Silicone Photodiode
Back-lit LCD 94W x 70H mm.
370W x 550D x 265H mm.
Net weight
20 kg.
Gross weight
25 kg.
Power requirements
110 – 220V selectable, 50/60 Hz
Ordering information:
Catalogue number

Halo RB-10 UV-Visible Ratio Beam Spectrophotometer 110 – 220V selectable, 50/60 Hz, 4mm. slit width, including 4 cell holder

Includes: #46-03-0007 Quartz cuvette (pack of 2)

Rectangular long-path cuvette holder (holds 4 x cuvettes)
Thermostatic cuvette holder with tubing
Test tube holder (includes high ceiling cover)
Micro-cuvette holder
Micro-cuvettes – quartz 50 μl / 10 mm. optical path length
Sample sipper
UV Detective™ software (see separate specifications)


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