Halo DB-30 UV / Visible Double Beam Spectrophotometer
The next generation Halo DB-30 double beam spectrophotometer boasts superlative performance characteristics for applications demanding the utmost sensitivity by combining higher signal to noise ratio with minimal stray light. This warrants the DB-30 suitable to many diverse fields and industries such as pharmaceutical, food, sanitation, environment, biology, agriculture, geology, mineral, petrochemical, optical, life science, education, manufacturing and many others.Exceptionally low stray light
The intricate but elegant and innovative optical pathway is engineered to deliver outstanding double beam performance characteristics, in particular stray light, which achieves an impressive low 0.01% T. The outcome is an increase in the linearity of absorbance characteristics for most samples thus increasing the scope of analytical applications particularly at higher concentrations.
Spectral Features
The asymmetric monochromator with long focal length results in improved sensitivity. Furthermore, up to 6 slit widths are selectable and switchable with the highest achievable resolution of 0.1 nm.) thus allowing for the optimisation of different applications.
Other specifications include an impressive wavelength accuracy of ± 0.3 nm., noise level 0.0003 Abs (500 nm.), wavelength repeatability of ± 0.1 nm. for the accuracy and repeatability of analytical data and an absorbance range of -4 to +5 Abs for the analysis of high absorbance samples without the need for further dilution.
High Scan Speeds and Resolution
Select from 8 scan speeds starting at a high resolving 1 nm. / minute. Coupled with the precise fine tuning of the photomultiplier tubes, a wavelength resolution of 0.1 nm. is maintained at even the fastest scan speed of 2,000 nm. / minute.
Powerful UV Detective PC Control
The Halo DB-30 is PC controlled using the UV Detective™ software (included with the instrument). Refer to separate section for further details on the UV Detective software. UV Detective can also operate optional accessories such as the sample sipper and 6-cuvette holder.
The UV Detective software (see separate specifications)is powerful but user friendly using Windows® XP Pro and Vista, as depicted in the process flow below:
1. Select function (e.g photometry)
2. Set method parameters
3. Auto-zero or baseline
4. Sample test
5. Data processing
6. Report generation
Dual Lamp Advantage
By virtue of the long life, halogen tungsten and deuterium lamps, the Halo DB-30’s wavelength range extends from 190 nm. – 1,100 nm. Furthermore, the dual lamp system results in higher accuracy than corresponding xenon lamps. Lamp switching is automatic and selectable from a wavelength range of 325 nm. to 370 nm.
Validation Functions
To ensure optimum instrument performance, a self-diagnostic function incorporating a number of parameters and wavelength calibration are automatically initiated upon start-up. Furthermore, the Halo DB-30 with the UV Detective™ software is equipped with a GLP / GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can be all validated and the audit report printed.
Halo UV Detective™ Software
Halo UV Detective™ is powerful, user friendly software specifically designed for the control (and data processing) of selected Halo spectrophotometers from computers installed with the Windows® XP Pro and Vista operating system.
The versatile UV Detective™ can control all spectrophotometer operations such as photometry, wavelength scans, time scans and more. Further functions include storage of methods programme, saving of numerical and graphical data, downstream data processing, data transfer to commercial spreadsheets such as Microsoft™ Excel and report generation.
Specifications:
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Compatible Spectrophotometers
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Halo RB-10 (optional), Halo DB-20 (optional), Halo DB-30 (standard)
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Control Functions
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Wavelength setting, auto-zero, auto calibration, optical path calibration, accessories such as 6-cuvette positioner and sipper
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Measurement Conditions
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Start-up, setting, output and storage of measurement parameters
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Measurement Function
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Wavelength scan, time scan, quantitative analysis, multi-spectrum measurement, kinetic analysis, concentration measurement and nucleic acid / protein measurements
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Data Output
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Display of spectra, data and scans (time and spectrum)
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Quantitative Methods
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Multi-wavelength, input of constant, standard curve calibration (linear, quadratic, cubic and segment)
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Data Processing
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Integral, derivative, flatness, calculation (spectrum and constant) and kinetic
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DB-30 Accessories
Rectangular long-path cuvette holder: Designed for low concentration or low absorbance samples
- Accomodates 2 x long-path cuvettes
- Accepts cuvettes with 6 optical path lengths of: 10, 20, 30, 40, 50 & 100 mm.
Cylindrical long-path cuvette holder: Designed for low concentration or low absorbance samples using a cylindrical cuvette.
- Accomodates 2 x long-path cylindrical cuvettes (sample and reference sides)
Thermostatic cuvette holder: Designed for applications requiring incubation and / or maintenance of a sample at a constant temperature.
- External water circulation maintains temperature stability
- Operating temperature range: ambient to +40°C
- Temperature stability: ± 0.3°C
- Complete with tubing for quick connection to water source (such as circulating water bath)
5-Cuvette holder / changer: Designed for mounting up to 5 standard 10 mm. cuvettes on the sample beam side.
- Total capacity: 5 cuvettes on sample beam side + 1 cuvette on reference beam side
- Turret design cuvette holder for efficient changeover
- Manual change of cuvettes by turning front mounted knob
- No temperature control
6-Cuvette holder / changer with electronic temperature control: Designed for mounting up to 6 standard 10 mm. cuvettes on the sample beam side with temperature control and stirring.
- Total capacity: 6 cuvettes on sample beam side + 1 cuvette on reference beam side
- Electronic changeover - change cuvettes automatically at defined time intervals
- Electronic thermostat – set temperature between +20°C to +40°C
- Includes magnetic stirrer (and fleas)
- Requires water circulated cooling (tubing included)
6-Cuvette holder / changer (without electronic temperature control): Designed for mounting up to 6 standard 10 mm. cuvettes without temperature control and stirring.
- Total capacity: 6 cuvettes on sample beam side + 1 cuvette on reference beam side
- Electronic changeover - change cuvettes automatically at defined time intervals
Micro-cuvette holder: Designed for measuring micro-volumes with 50μl. micro-cuvette.
- Wavelength range: 220 to 880 nm.
- Noise level: - 0.001 Abs (with 50μl. volumes)
Micro-cuvettes: Suitable for use in the micro-cuvette holder.
- Made from quartz
- Available size: 50μl.
Auto Sample sipper (without temperature control): Designed for the rapid measurement of multiple or large amounts of sample without the requirement for manual washing or changing of cuvettes. Sample is sipped from an external tube directly into the sipper’s integrated cuvette and automatically measured. The sample can also be recovered post-measurement.
- Minimum sample volume: 0.7 ml.
- Wavelength range: 190 nm. – 900 nm.
- Carryover: ≤ 1%
- Sipper cuvette capacity: approx. 50μl.
- Optical path length: 10 mm.
Auto Sample sipper (with temperature control): The same features as the Auto sipper with the added convenience of electronic temperature control to maintain the flow cuvette at a constant temperature.
- Temperature control range: +20° to +40°C
- Requires water circulated cooling (tubing included)
Micro Flow Cuvette Holder
Designed for the continuous measurement of trace samples. The sample can be injected into the flow cuvette with a syringe or other injection device.
- Flow cuvette capacity: 70 μl.
- Pressure tolerance: Max. 0.1 Mpa
- Optical path length: 10 nm.
- Teflon tubing provided
Glass Sample Holder
Designed for measuting the transmittance / absorbance of glass samples or filters.
- Glass sample thickness: 0.5 mm. to 5 mm.
- Glass sample dimensions: Min. 12 x 25 mm. to Max. 55 x 100 mm.
Film Sample Holder
Designed for measuting the transmittance / absorbance of thin film-like samples.
- Film sample dimensions: 25 mm. (W) x 30 – 50 mm. (H)
- Beam aperture: 10 mm. (W) x 20 mm. (H)
Specifications:
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Optics
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Concave diffraction grating / Double beam principle
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Wavelength range
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190 nm. – 900 nm.
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Spectral bandwidth
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Selectable: 0.1 nm., 0.2 nm., 1.0 nm., 2.0 nm., 5.0 nm.
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Stray light
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≤ 0.01% (220 nm. NaI, 340 nm. Na NO2)
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Wavelength accuracy
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± 0.3 nm.
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Wavelength repeatability
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± 0.1 nm.
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Setting wavelength
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0.01 nm. increments
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Photometric Accuracy (NIST 930D Filter)
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± 0.002 Abs ( 0 – 0.5 Abs)
± 0.004 Abs ( 0.5 – 1 Abs)
± 0.008 Abs ( 1 - 2 Abs)
± 0.3% T
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Photometric Repeatability (NIST 930D Filter)
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± 0.001 Abs ( 0 – 0.5 Abs)
± 0.002 Abs ( 0.5 – 1 Abs)
± 0.004 Abs ( 1 - 2 Abs)
± 0.15% T
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Measurement Modes
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Abs, %T, Conc., E(S), E(R)
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Photometric range
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Absorbance: -4 to +5
Concentration: -9.999 to +9.999
E(S), E(R): 0 ~ 600
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Wavelength scan speed
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Selectable: 1, 5, 20, 120, 300, 1000, 1600, 2000 nm. / minute
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Wavelength slew speed
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3000 nm. / minute
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Baseline flatness
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± 0.001 Abs (200 – 850 nm.)
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Baseline stability
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0.0004Abs / hr. (500 nm. after 2 hours)
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Noise level
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0.0003 Abs (500 nm.)
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Light source
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Tungsten-Halogen and Deuterium lamps
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Light source switching
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Automatic switching selectable from 325 nm. to 370 nm..
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Detector
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Photomultiplier
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Instrument Control
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PC with Windows® XP Pro operating system
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Dimensions
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710W x 630D x 268H mm.
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Net weight
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50 kg.
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Gross weight
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56 kg.
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Power requirements
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220V AC, 50/60 Hz, 300 VA
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Ordering information:
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Catalogue number
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Description
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DB-30-220
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Dynamica Halo DB-20 UV-Visible Double Beam Spectrophotometer 220V AC, 50/60 Hz with UV Detective™ software (see separate specifications) Includes: #46-03-0007 Quartz cuvette (pack of 2) and #46-03-0001 Glass cuvette (pack of 4) |
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DB-30-RLPH
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Rectangular long-path cuvette holder
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DB-30-CLPH
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Cylindrical long-path cuvette holder
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DB-30-TCH
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Thermostatic cuvette holder with tubing
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DB-30-FCC
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Manual 5-cuvette holder / changer
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DB-30-SCCT
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Auto 6-cuvette holder / changer with temperature control and stirrer
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DB-30-SCC
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Auto 6-cuvette holder / changer without temperature control and stirrer
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DB-30-MCH
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Micro-cuvette holder
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DB-30-MC-50
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Micro-cuvettes – quartz 50 μl / 10 mm. optical path length
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DB-30-SST
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Auto sample sipper with temperature control
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DB-30-SS
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Sample sipper without temperature control
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DB-30-MFH
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Micro flow cuvette holder
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DB-30-GSH
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Glass sample holder
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DB-30-FSH
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Film sample holder
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